Phytochemistry: An in-silico and in-vitro Update by Shashank Kumar & Chukwuebuka Egbuna

Author:Shashank Kumar & Chukwuebuka Egbuna
Language: eng
Format: epub
ISBN: 9789811369209
Publisher: Springer Singapore

14.4 Conclusion

In summary, rapid progress has been made in the development of in-vitro methodologies to understand and explore the biochemical pathway both in plants and animals. However, many of the present experimental procedures document signal transduction snapshots of individual events, without the benefit of visualizing the whole stage simultaneously. The emergence of newer assays using fluorescently tagged transfected proteins combined with live cell imaging analysis are useful to confirm and modify current perceptions of signal transduction events. Nonetheless, future experiments designed to dissect signal transduction pathways and their morphologic effects will require a combination of all of these approaches and surely new ones. The reemergence of FRET and FRAP to visualize living cells, use of transgenic models in both plant and animals has revolutionized how signal transduction events are understood. Further, innovation in the procedure utilizing custom fusion proteins, transfection of engineered proteins into embryonic tissues to view signal transduction events in whole tissues are some of the area which needs to be explored further. Also, methodologies for rapid detection of phosphorylation, dephosphorylation, protein kinase action, is essential and should always expect to uncover some newer methodologies and techniques to improve and better understand this intense network of signaling.

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